transfection efficiency

RESULTS: The transfection efficiency of siRNA was 81%.

结果：相差显微镜荧光照片显示细胞转染的效率为81%。

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Then transfection efficiency was observed under fluorescence microscope.

以脂质体法转染PC-3细胞株后，在荧光显微镜下观察转染效率。

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The fluorescence photos showed that the transfection efficiency of HT-29 cells was 60%. 2.

结果：1.荧光显微镜照片fluorescence显示大肠癌HT-29细胞的转染效率为60%。

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When N/ P ratio equaled to 5, the highest transfection efficiency of Tf-PEI was obtained.

当N/P比值为5时，Tf-PEI的转染效率最高。

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The transfection efficiency of pentaerythritol-based cationic liposomes which have the same hydrophobic chain was reduced with the increase of the hydrophilicity. And the optimal length of hydrophobic chain of the trimethylolpropane-based cationic liposomes is tetradecyl.

三羟甲基丙烷类阳离子脂质体最佳疏水链为十四烷基；疏水链长度相同，脂质体的转染效率随亲水性增强而升高。

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Additionally, PEI is considered to exhibit high transfection efficiency and endosomal disrupting capacity.

此外, PEI也被认为具有高转染率和使内涵体破裂的能力.

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Effect of Different Transfection Methods and Green Fluorescent Protein Expression Vectors on Transfection Efficiency in Mouse Embryonic Stem Cell

不同转染方法及GFP表达载体对小鼠胚胎干细胞转染效率的比较

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When optimal MOI is 100, transfection efficiency is above 90%.

最适感染复数为100，转染率可达90%以上。

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Transfection efficiency was detected by fluorescence microscopy.

荧光显微镜观察转染效率，RT-PCR筛选出RNA干扰效果最好的重组质粒。

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Based on these facts, chitosan can be used as a condensing agent to enhance the transfection efficiency of cationic liposome.

在上述基础上，研究了运用壳聚糖作为压缩基团来提高阳离子脂质体介导的基因传递。

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Cationic liposome transfection efficiency on human renal cell carcinoma cell line

人肾细胞癌细胞阳离子脂质体的转染效率

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Enhancive Effect of Histone Deacetylase Inhibitor Trichostatin A on Transfection Efficiency of Adenovirus in Ovarian Carcinoma Cell Line A2780

HDAC抑制剂TSA增强卵巢癌细胞株A2780对腺病毒基因转染效率的体外研究

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Although recombinant viral vectors have high transfection efficiency, they are associated with immunogenicity, toxicity, lack of tissue specificity, unknown long-term effects et al.

虽然病毒载体具有较高的转染效率，但同时也存在着很多难以克服的缺点，如具有免疫原性、细胞毒性、缺少组织特异性等等。

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Conclusion Tf can enhance gene-liposome transfection efficiency, furthermore with combination of interventional catheter technique, there would be a potential duel-target-orientated gene therapy method. ( J Intervent Radiol, 2007,16:99-103)

结论Tf能增强脂质体-基因转染，介入技术与Tf联合双重靶向对肝癌的基因治疗具有相当的应用前景。

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